Saturday, April 13, 2013

A lab report on the investigation of temperature on catalase activity.

INVESTIGATION INTO THE EFFECT OF TEMPERATURE ON CATALASE ACTIVITY

AIM

The aim of this experiment is to find erupt the effect of temperature on catalase or hydrogen peroxide. This will alter us to tell at what temperature hydrogen peroxide is roughly efficient. This (degradation) reaction will help us determine around of the catalases different attributes.

HYPOTHESIS

In this experiment it would be safe to hypothesise that no activity would pass on place at 1 to 20 degrees. It would be probable that a little activity would take place around 40 degrees. When the piss gets to around 60+ the oxygen bubbles start to froth.

It is almost certain that this will overhaul due to the behaviour of the atom. This means that the hotter the water gets the more mobile the water molecules become. Thus the colder the water gets the less officious the water becomes. Another theory is that different things adapt according to their habitat. Therefore, catalase would be most active at around 37 degrees consistency temperature. This means that in this experiment catalase would be most active in the water bath that is at 40 degrees Celsius.

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METHOD

Before the experiment could be started the following apparatus needed to be obtained:

Cork Borer

Water Baths at 3,20,40 and 60 degrees Celsius

Potato

Ruler

Stopwatch

Detergent

Hydrogen Peroxide

Now that all this apparatus has been obtained, it is possible to start the experiment. ogdoad block out tubes will be required because the experiment is creation done twice in each water bath.

Use a marker pen to mark the specific temperature on the test tubes so as to make sure that there atomic number 18 no possible errors. Now place these test tubes in a test tube rack. Take the cork rock drill no. 4, and take a cylinder of potato victimization the cork...

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